Evolution-guided protein design of IscB for persistent epigenome editing in vivo.
Europe PMC REST API · 學術索引收錄 · 來源發佈：Wed May 07 2025 00:00:00 GMT+0000 (Coordinated Universal Time) · 本站收錄：Thu Oct 08 2026 09:08:13 GMT+0000 (Coordinated Universal Time)
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Naturally existing enzymes have been adapted for a variety of molecular technologies, with enhancements or modifications to the enzymes introduced to improve the desired function; however, it is difficult to engineer variants with enhanced activity while maintaining specificity. Here we engineer the compact Obligate Mobile Element Guided Activity (OMEGA) RNA-guided endonuclease IscB and its guidin
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